Estimation of total antioxidant capacity in type 2 diabetic and normal healthy subjects
Keywords:Total Antioxidant Capacity (TAC), Type 2 Diabetes Mellitus (DM), BMI, ELISA, Cardiovascular Disease (CVD).
Background: Diabetes mellitus (DM) is a highly prevalent non-communicable disease in the world. Current investigations evolved that oxidative stress is also a major risk factor to cause type 2 diabetes mellitus due to impairment of antioxidant defense system in various biological fluids.
Methodology: In this cross-sectional study, 70 type 2 diabetes mellitus subjects and 30 normal healthy subjects of both genders were selected from various health care centers of Karachi, Pakistan for a study period of six months June 2017 – December 2017. The total antioxidant capacity (TAC) concentration was measured in serum by enzyme-linked immunosorbent assay (ELISA) technique using Caymans Antioxidant Assay. The biochemical parameters and anthropometric measurements were estimated by standardized methods. Data was analyzed using the statistical program Statistical Package for the Social Sciences (SPSS) version 10.0.
Results: According to the study results TAC was significantly reduced (**0.05 ± 0.00 mmol /L) in type 2 diabetes mellitus subjects compared to normal healthy subjects (0.13 ± 0.02 mmol /L). It was noted that diastolic blood pressure (DBP), body mass index (BMI), and triglycerides (TG’s) were significantly increased while high density lipoprotein-cholesterol (HDL-C) was significantly reduced in diabetic subjects than the comparative healthy individuals.
Conclusion: This study showed that decreased levels of TAC and HDL-C in type 2 DM patients with increased levels of BMI, systolic blood pressure (SBP), fasting blood sugar (FBS), DBP, and total cholesterol (TC) which may cause oxidative stress and increase the progression of cardiovascular disease (CVD) and other metabolic diseases. Modifications in dietary habits and intake of antioxidant foods or supplements may diminish the process of oxidative stress which may consequently decrease CVD and other severe clinical outcomes.