International Journal of Endorsing Health Science Research (IJEHSR) <p><strong>International journal of endorsing health science research (IJEHSR)</strong>&nbsp;aims to&nbsp;improve health care by providing the most recent research results&nbsp;leading to advancement in health science.</p> <p><strong>IJEHSR</strong>&nbsp;is quarterly, open access, peer-reviewed&nbsp;journal keen to endorse the need for finest healthcare globally, it was founded in 2013 as a biannual journal, and converted into quarterly in 2015. &nbsp;<strong>IJEHSR</strong>&nbsp;is included in the journal list of International Committee of Medical Journal Editors (ICMJE) since 2014. This Journal is indexed by WHO Index Medicus (IMEMR) for the Eastern Mediterranean Region.&nbsp;<strong>IJEHSR</strong>&nbsp;editorial board members are working in diverse fields of health care in countries like&nbsp;<strong>Pakistan, Canada, Australia, United States of America, China, India, Oman,&nbsp;Saudi&nbsp;Arabia,&nbsp;</strong>and<strong>&nbsp;Ethiopia.</strong></p> <p>We would like to encourage you to submit your intellectual effort in&nbsp;IJEHSR, we consider manuscripts from all fields of health sciences.&nbsp;The journal publishes original research, reviews, clinical reports, case studies, legal and policy perspectives.</p> en-US <p>By submitting a manuscript in&nbsp;<strong>IJEHSR</strong>, the Author (Authors if a multi-authored paper) confirms all the clause of the <a href="">Copyright Notice</a></p> (Editorial Office) (Managing Editor) Wed, 27 Apr 2022 00:00:00 +0000 OJS 60 Knowledge and perception of doctors regarding antibiotic stewardship in a tertiary care hospital of Southern Punjab. <p><strong>Background:</strong> Pakistan has been working on Antimicrobial Resistance (AMR) for a decade; unfortunately, there is a lack of concept of antibiotic stewardship in most health setups, especially in the public sector. This study aims to analyze the knowledge and perception of junior physicians towards antibiotic stewardship programs. The need for this knowledge and impact of antimicrobial resistance on antibiotic stewardship ascertain barriers to stewardship acceptance.</p> <p><strong>Methodology:</strong> A cross-sectional study was conducted at different clinical departments of Sheikh Zayed Hospital, Rahim Yar Khan, including 50 junior physicians recruited via convenience sampling technique. The data was collected using a structured questionnaire comprising physician's hospital associated data and questions regarding the knowledge, perspectives, and practices concerning antibiotic stewardship programs (ASP) to reduce AMR. Statistical analysis was done using SPSS version 22.0, and data were presented using frequencies and percentages.</p> <p><strong>Results:</strong> Most of the enrolled physicians knew AMR and agreed that it is a serious global health issue. However, all the medical officers were completely unaware of the antibiotic stewardship program, while 42.9% of house officers and only 25.7% of PGs knew about the program.</p> <p><strong>Conclusion:</strong> Our study shows support from doctors for expanded stewardship implementation and provides an important understanding of the current attitudes of doctors regarding stewardship execution. A better understanding of perceptions and attitudes is dire for healthcare stakeholders to expand stewardship activities into healthcare settings.</p> Moazzam Ali Atif, Sana Tufail ##submission.copyrightStatement## Mon, 09 May 2022 10:42:23 +0000 Effects of anti-tuberculosis drugs on lipid profile in pulmonary tuberculosis patients <p><strong>Background:</strong> Complement activation occurs in the vessel's wall as serum lipids start to get deposited, marking the beginning of atherosclerosis formation. Carotid intima-media thickness (IMT) is a widely used tool for detecting atherosclerosis. This study objective was to correlate lipid profile with C3d levels and Carotid IMT in type 2 Diabetic patients.</p> <p><strong>Methodology:</strong> This case-control study comprised 120 male and female participants between 35 to 65 years of age. Of them, 90 type 2 diabetes patients were stratified according to the diabetes duration into four groups, Group A (n=30) &lt;5 years of diabetic, Group B (n=30) 5-10 years of diabetic, Group C (n=30) &gt;10 years diabetic and non-diabetic subjects healthy controls in Group D (n=30). The Anthropometric measurements (height, weight, and BMI), serum C3d level, carotid intima-media thickness (carotid IMT) &amp;, serum levels of Cholesterol, Triglycerides, Low-density lipoprotein (LDL), and High-density lipoprotein (HDL) were evaluated.</p> <p><strong>Results:</strong> In Type 2 Diabetic patients with moderate to high levels of serum Cholesterol, the C3d levels were found to be significantly raised (10.58 ±3.87 µg/ml and 15.37 ± 2.51 µg/ml, respectively), but Carotid IMT was increased in patients with high Cholesterol (0.98 ± 0.11 mm). Patients with moderate to high levels of Triglycerides had C3d 11.03 ± 3.85 µg/ml and 13.66 ± 4.30 µg/ml, respectively, but Carotid IMT was observed to be raised in patients with higher triglycerides levels (1.00 ± 0.11 mm). Also, in patients with moderate to high serum LDL C3d were 10.26 ± 3.10 µg/ml and 13.14 ± 4.08 µg/ml, respectively, whereas Carotid IMT was increased in patients with high levels of LDL (0.93 ± 0.13 mm). The C3d (12.12 ± 4.42 µg/ml) and Carotid IMT (0.90 ± 0.13 mm) levels were increased as HDL decreased significantly.</p> <p><strong>Conclusion:</strong> Serum C3d can be used as an early marker of atherosclerosis formation, which increases as lipid levels exceed the normal range in diabetic type 2 patients compared to carotid IMT detected in patients with higher lipid parameters.</p> Sana Soomro, Nargis Anjum, Nosheen Wasi, Uzma Najm, Nuvair Zia, Adil Ramzan ##submission.copyrightStatement## Mon, 09 May 2022 11:22:54 +0000 Isolation and identification of Murine Mesenchymal Stem Cells from Bone Marrow. <p><strong>Background:</strong> Mesenchymal stem cells (MSCs) are beneficial for cell and gene therapy approaches. These cells are highly potent in producing various cells, including adipocytes, osteoblasts, hematopoietic cells, etc., particularly bone marrow-derived. High proliferation and regenerative capacity are important phenotypes of MSCs which contribute to the recovery process at the damaged sites. Various techniques have been used, which have inconvenient effects on stromal cell properties. The present study evaluates the stem cell markers in the isolated cells from the Murine mouse bone marrow.</p> <p><strong>Methodology:</strong> The murine mesenchymal stem cells (mMSCs) were isolated from the bone marrow of murine Balb/c mice using a simple in vitro culture protocol. Trypan blue exclusion assay was performed for viable cell count, and cellular morphology was checked using an inverted microscope. The immunofluorescence staining was done to analyze the immunophenotypic features of mMSCs by treating cells with CD44, CD90, and CD45 fluorescent antibodies.</p> <p><strong>Results:</strong> mMSCs with spindle-like cell appearance were attained within three weeks. Immunofluorescence staining of isolated mMSCs displayed a statistically significant (p&lt;0.001) number of CD44 and CD90 positive cells. Alternatively, an insignificant count of CD45 labeled cells was found.</p> <p><strong>Conclusion:</strong> A uniform mMSC population was achieved with high propagation capability in lower passages through regular replacement of medium and reduced trypsinization time. In addition, mMSCs were strongly expressed MSCs positive markers, including CD44 and CD90.</p> Sehrish Jabeen, Muhammad Sufyan Vohra, Yasir Raza ##submission.copyrightStatement## Mon, 16 May 2022 08:33:49 +0000 Effect of hydroxyurea on quality of life with a moderating role of healthcare professionals’ performance: A view from the beta-thalassemia patients. <p><strong>Background:</strong> Transfusion-dependent (TDT) β –Thalassemia patients have lower HRQoL than healthy individuals. The impact of Hydroxyurea is always measured with the clinical investigations in terms of laboratory diagnosis. This study aimed to seek out the impact of the drug Hydroxyurea on improving physical health problems of Quality of Life (QoL) of β – Thalassemia patients with the moderating role of healthcare professionals.</p> <p><strong>Methodology:</strong> In this research, a deductive approach was adopted whereby hypotheses were originated first, followed by organizing a well-structured questionnaire as a tool for measuring the data. The nature of the research was explanatory and based on primary quantitative data. The data was collected from 290 patients visited in the outpatient and daycare departments of the National Institute of Blood Diseases and Bone Marrow Transplantation Hospital.</p> <p><strong>Results:</strong> This study revealed that the impact of Hydroxyurea therapy on improving the physical health problems of Beta Thalassemia is not evident as per the analysis (r=0.079; p=0.178). Similarly, a weak positive non-significant correlation exists between Hydroxyurea therapy and the performance of healthcare professionals in the hospital (r=0.016; p=0.782). Moreover, no significant improvement was observed in physical health problems and health domain of QoL of BT patients by moderating the role of healthcare professional performance (p=0.4471 and 0.4102, respectively).</p> <p><strong>Conclusion: </strong>It is concluded that moderating the role of healthcare professionals' performance does not improve the impact of Hydroxyurea on physical health problems and domains of QoL of β – Thalassemia patients.</p> Affaf Sheikh, Munaza Bibi, Saima Siddiqui, Kousar Perveen, Tahir Shamsi ##submission.copyrightStatement## Tue, 17 May 2022 10:31:39 +0000 Screening of different doses of lyophilized beetroot for their hematopoietic potential. <p><strong>Background:</strong> Beetroots are considered a vital source of nutrition and are usually incorporated into the daily diet to prevent different disease conditions. The therapeutic effect observed is due to the presence of active ingredients in the vegetables. The majority of our population, especially females, suffers from anemia due to malnutrition. The perseverance of this research was to explore the hematopoietic effects of lyophilized beetroot powder at different doses so they could be recommended as a nutritional supplement.</p> <p><strong>Methodology:</strong> Albino rabbits weighing 1500-2000 gm of either gender were selected for the study and alienated into three sets. Set I was administered distilled water, whereas sets II and III were given lyophilized beetroot 500 mg/kg and 1000 mg/kg, correspondingly. For hematopoietic evaluation of blood, automated huma count plus was utilized. After adjusting according to their weights, lyophilized beetroot powder was suspended in distilled water and given at 500 mg/kg and 1000 mg/kg to rabbits. They were dosed once daily for two months.</p> <p><strong>Results:</strong> The effects were verified before the dosing was scheduled and on the 30th day and 60th day of dosing. The outcomes presented a highly noteworthy increase (p&lt;0.0001) in hemoglobin, erythrocyte count, hematocrit, leukocyte count, and thrombocytes by both doses. 500 mg/kg dose showed a more significant increase in leukocytes and thrombocyte count compared to 1000 mg/kg, whereas 1000 mg/kg showed a more substantial increase in erythrocytes and Hb as compared to 500 mg/kg at the conclusion of the study period.</p> <p><strong>Conclusion:</strong> From the above, it can be concluded that beetroot possesses pronounced hematopoietic effects and might be beneficial as a non-pharmacological treatment for anemia and thrombocytopenia.</p> Sana Sarfaraz, Rahela Ikram, Rabia Munawwar, Sabiha Gul ##submission.copyrightStatement## Thu, 19 May 2022 10:01:54 +0000 Rapid Screening: Post Computerized Tomography Incidence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Features in Asymptomatic Patients <p><strong>Background:</strong> It is to document the frequency of incidental Corona Virus Disease-2019 (COVID-19) specific findings in patients imaged after the initial ten days of lockdown relief and the sensitivity of computed tomography (CT) in picking unknown ongoing disease processes.</p> <p><strong>Case presentations:</strong> In this study, we presented seven cases—three cases in plain CT pyelogram and four cases of post-contrast scans. Post-contrast scans of chest findings in case 1 showed few pre-vascular, pre-tracheal, and sub-carinal lymph nodes with calcified granuloma in the left lower zone accompanied by extensive bilateral peripheral Ground Glass Haze with alveolar opacification. In Case 2, multiple non-homogeneous areas of ground-glass haze diffusely involving both lung fields were seen. Case 3 showed a few sub-centimeters mediastinal lymph nodes with diffuse mosaic attenuation patterns in both lung fields along with multiple soft tissues density nodules in both lungs. While in Case 4, Patchy areas of alveolar opacification with surrounding ground-glass haze were noted in lower lobes.</p> <p><strong>Management and Results:</strong> Total 64 CT C/A/P (chest/abdomen/pelvis) with contrast scans and 120 plain pyelograms were done within ten days after lockdown relief, and it was observed that during the stricken lockdown, no incidental case of positive CT finding was observed. However, afterward, within this time, 44 cases (23.9%) were recorded in asymptomatic patients of COVID-19.</p> <p><strong>Conclusions:</strong> CT chest is highly sensitive in detecting COVID-19 with high accuracy of featuring progress in the disease and associated findings even in the initial stages.</p> Syed Muhammad Faiq, Syeda Nuzhat Zehra, Syeda Sukaina Jafri, Sumreen Begum ##submission.copyrightStatement## Wed, 27 Apr 2022 00:00:00 +0000