International Journal of Endorsing Health Science Research (IJEHSR) <p><strong>International journal of endorsing health science research (IJEHSR)</strong>&nbsp;aims to&nbsp;improve health care by providing the most recent research results&nbsp;leading to advancement in health science.</p> <p><strong>IJEHSR</strong>&nbsp;is quarterly, open access, peer-reviewed&nbsp;journal keen to endorse the need for finest healthcare globally, it was founded in 2013 as a biannual journal, and converted into quarterly in 2015. &nbsp;<strong>IJEHSR</strong>&nbsp;is included in the journal list of International Committee of Medical Journal Editors (ICMJE) since 2014. This Journal is indexed by WHO Index Medicus (IMEMR) for the Eastern Mediterranean Region.&nbsp;<strong>IJEHSR</strong>&nbsp;editorial board members are working in diverse fields of health care in countries like&nbsp;<strong>Pakistan, Canada, Australia, United States of America, China, India, Oman,&nbsp;Saudi&nbsp;Arabia,&nbsp;</strong>and<strong>&nbsp;Ethiopia.</strong></p> <p>We would like to encourage you to submit your intellectual effort in&nbsp;IJEHSR, we consider manuscripts from all fields of health sciences.&nbsp;The journal publishes original research, reviews, clinical reports, case studies, legal and policy perspectives.</p> en-US <p>By submitting a manuscript in&nbsp;<strong>IJEHSR</strong>, the Author (Authors if a multi-authored paper) confirms all the clause of the <a href="">Copyright Notice</a></p> (Editorial Office) (Managing Editor) Wed, 31 Mar 2021 10:20:43 +0000 OJS 60 Comparison of visual outcome in senile cataract after phacoemulsification and manual small incision cataract surgery. <p><strong>Background: </strong>Different surgical treatment options are used to treat cataracts, such as Manual Small Incision Cataract surgery (MSICS) and Phacoemulsification. The present study compares the visual outcomes of phacoemulsification and MSICS in senile cataract patients.&nbsp;</p> <p><strong>Methodology:</strong><strong>&nbsp;</strong>A quasi-experimental study was conducted with 270 patients aged between 50 and 70. Patients diagnosed with senile cataract were included in the study and divided equally into two groups, Group A patients underwent MSICS, and Group B patients underwent Phacoemulsification. Pre-operative visual acuity and Postoperative visual acuity were assessed between the two groups on the 1<sup>st</sup> day, 1<sup>st</sup> week, and 1<sup>st</sup> month.</p> <p><strong>Results:</strong><strong>&nbsp;</strong>Significant difference (p=0.001) was observed on 1<sup>st</sup> day of assessing uncorrected visual acuity among both interventional groups. However, no significant difference was observed in uncorrected visual acuity postoperatively on the 1<sup>st</sup> week (p=0.093) and 1<sup>st</sup> month (p=0.266).</p> <p><strong>Conclusion: </strong>Both Phacoemulsification and MSICS are beneficial surgical options to treat senile cataract, with both showing similar efficacy to one another.</p> Irshad Ahmed Bhutto, Irshad Ali, Maria Nazish Memon, Attaullah Shah Bukhari, Ikramuddin Indhar, Nasir Ahmed Memon ##submission.copyrightStatement## Wed, 31 Mar 2021 10:18:51 +0000 Isolation of Vibrio cholerae from clinical and drinking water samples during Cholera Outbreak in Khairpur Sindh Pakistan <p><strong>Background:</strong> Cholera is a diarrheal disease that is caused by the bacterium Vibrio cholerae that transmits through contaminated water, food, oral-fecal route and spread through poor sanitation system. This study aims to investigate the reports of clinical and drinking water samples during an epidemic in Pakistan.</p> <table width="100%"> <tbody> <tr> <td> <p>Accepted Article <strong>|</strong> This is not yet the definitive version of record, it is to give early visibility of the article<strong> |</strong> An open access article published ahead of print under the CC BY license (<a href=""></a> <strong>| </strong>DOI and Page numbers will be assigned to the final version before inclusion into the next issue</p> <p>&nbsp;</p> <p>&nbsp;</p> </td> </tr> </tbody> </table> <p><strong>Methodology:</strong> This study was conducted in District Khairpur including 660 samples (360 from clinical and 300 from drinking water) from 2014-2016. All samples were enriched in Alkaline peptone water for 6 hours and then streaked on TCBS agar, incubated at 35ºC for 24 hours. The next day standard microbiological, biochemical, serological techniques were used for the identification of V. cholerae and further identification was performed using PCR.</p> <p><strong>Results:</strong> Out of 360 clinical samples, 76(21.11%) were positive for V. cholerae. The species-specific Outer membrane protein W precursor (ompW) gene was amplified and shown at the correct size (588 bp) through agarose gel electrophoresis. The serotyping revealed that the isolates belonged to serogroup Inaba, O1. cholerae O1 strains shown typical El Tor phenotype similar to V. cholerae El Tor strain N16961 (PBR VP+) used as the reference strain in this study. All age groups were affected where the highest onset was seen among those aged 19 years and above. The culture of drinking water (n=300), observed negative on TCBS agar.</p> <p><strong>Conclusion:</strong> As far as our knowledge, this is the first time when the presence of V. cholerae El Tor peak has been reported for causing cholera outbreaks in Khairpur Sindh, Pakistan. However, these findings can be used for further investigations and recognizing control measures.</p> Amjad Ali Mughal, Yasmeen Faiz Kazi, Habib Bokhari ##submission.copyrightStatement## Sun, 28 Mar 2021 00:00:00 +0000 Transurethral Resection of Prostate and Blood loss: Efficacy of Dutasteride in reducing perioperative blood loss. <p><strong>Background:</strong> Benign Prostatic Hyperplasia (BPH) is the most common urological cause of urinary obstruction, affecting men above 50 years of age. Medical therapy used for managing BPH includes various medical regimes, including 5-alpha reductase inhibitors (5ARI), namely Dutasteride and more. The study aimed to evaluate the efficacy of four weeks' prior treatment with dutasteride on per-operative blood loss in patients of BPH undergoing transurethral resection of the prostate.</p> <p><strong>Methodology:</strong> A prospective observational study was conducted from January to December 2019 at the Kidney Centre, Karachi, Pakistan. A total of 64 patients were included and divided into two groups. Group A patients were those who had been taking dutasteride (0.5 mg/day) for four weeks or more before surgery, and group B patients were not taking dutasteride drug before surgery. All patients underwent standard TURP, and the intra-operative blood loss was calculated. The collected data was analyzed using SPSS version 22.0.</p> <p><strong>Results:</strong> In our study, there was a significantly less hemoglobin drop (1.2 gm vs. 2.2 gm) during prostate surgery in patients using dutasteride than those who were not using dutasteride.</p> <p><strong>Conclusion:</strong> This study has revealed that the use of dutasteride four weeks before surgery significantly reduces intra-operative bleeding.</p> Shakeel Haseeb uddin Siddique, Salman El Khalid, Muhammad Shoaib Mithani, Abdul Wasay Mahmood, Shariq Anis Khan, Fakhir Yousuf ##submission.copyrightStatement## Wed, 14 Apr 2021 00:00:00 +0000 In vitro Characterization of Enterococcus faecium strain LCM08 as a probiotic <p><strong>Background:</strong> Under the present situations, where health improvement has become the major concern, food supplements, augmenting health is a matter of interest. Presently, the concept of "functional foods" possessing medicinal attributes has been reborn as probiotics. Acid-tolerant and Gram-positive Lactic acid bacteria (LAB), being the major class of probiotics, possess health-promoting characteristics, provide better health with a robust immune system, and treat various digestive and neurological disorders. Hence, the aim of the present study was the isolation and identification of LAB.</p> <p><strong>Methodology:</strong> The LAB were isolated from various sources, including food products and clinical samples. <em>Enterococcus faecium</em> LCM08 being the most potent isolate tested for probiotic traits, including viability under acidic conditions, biological concentrations of bile salts and enzymes.</p> <p><strong>Results:</strong> A total of fifty-four strains were isolated, of which 7.2% showed broad-spectrum inhibition against the tested pathogens. Amongst them, <em>Enterococcus faecium</em> LCM08 owing to its broad-spectrum antagonism, when tested for its ability to withstand acidic conditions, displayed 63.58% viability and 72.19% survivability in the presence of bile salts. Moreover, strain sustained in the presence of digestive enzymes including protease (94.65%), trypsin (93.57%) and pancreatin (75.0%) as well as exhibited growth at temperatures ranging between 25˚C and 30˚C where the maximum viable counts recorded as 95% and 96.4%. The strain also produced antimicrobial metabolites at neutral pH. However, elevated temperatures (45˚C-50˚C) inhibited the production of bacteriocin-like inhibitory substances.</p> <p><strong>Conclusion:</strong> The preliminary investigations revealed that <em>Enterococcus faecium</em> LCM08 possesses the key attributes pre-requisite for a probiotic candidate.</p> Mariam Zia, Talat Y. Mujahid, Mamona Mushtaq, Muneera Naz Baloch ##submission.copyrightStatement## Sat, 17 Apr 2021 08:47:41 +0000 Antimicrobial evaluation of modified herbal and allopathic dental rinse solution against virulent strains. <p><strong>Background:</strong> Mouthwashes are antiseptic solutions and produce anti-inflammatory properties against periodontal infections in humans.&nbsp; Several in-vitro studies have been performed against virulent strains, as evidenced by an appropriate knowledge about their quality, effectiveness and safety in this research. This study is designed to test the antimicrobial effects and evaluation of mouthwashes against pathogens involved in dental caries and oral infections.</p> <p><strong>Methodology:</strong> Rinse solution (Mouthwash) has been developed by three different formulations.&nbsp; Anti-caries solutions were applied and checked against virulent bacterial and fungal pathogens. The antimicrobial effectiveness was identified using the agar well diffusion method and minimum inhibitory concentration (MIC).</p> <p><strong>Results:</strong> Chemisol showed potential killing activity and a zone of inhibition against pathogens ranging in 21-30 mm, which comparatively have higher zones than biosol and herbisol. Biosol also indicated efficacy within 11-13 mm zone of inhibition against <em>Salmonella typhi ATCC-14028, Salmonella enterica ATCC-6017</em> resistant to herbisol and chemisol. Moreover, biosol exhibited greater MIC against most microorganisms at direct concentration and 1:10 dilution compared to herbisol and chemisol.</p> <p><strong>Conclusion:</strong> The significant difference observed in the antimicrobial activities of herbal, herbal &amp; allopathic combination and allopathic dental washes against pathogenic strains. This study concluded that proper gargling with mouth wash solutions reduces the risk of life-threatening strains from fecal water &amp; food.</p> Mariyah Yacoob Bawa, Tanveer Abbas, Afsheen Aqeel, Saima Asif, Saeeda Bano ##submission.copyrightStatement## Thu, 22 Apr 2021 06:41:23 +0000 Fungal Infection Exacerbate Nasal Polyposis: A study conducted at KGN Teaching Hospital Bannu-KPK. <p><strong>Background:</strong> Nasal polyps were believed to be small tumours of nasal or sinus mucosa. With progressing research, they are associated with enlargement or swelling of nasal or sinus mucosa, allergy, asthma, any infection, sensitivity to aspirin, and/or cystic fibrosis unclear etiology. The present study aims to evaluate the prevalence of fungal infections that can result in the onset of nasal polyposis in the residents of district Bannu, Pakistan.</p> <p><strong>Methodology:</strong> A cross-sectional study was conducted at the ENT Department of Khalifa Gul Nawaz (KGN) teaching hospital in Bannu from March 2018 to February 2020, including 180 polyposis patients with the presence or absence of any fungal infestation. The subjects possessing mass other than nasal polyp based on clinical diagnosis were excluded from the study. The fungal infestation was observed with nasal endoscopy, and the patients were hospitalized for functional endoscopic sinus surgery (FESS). The staging of the nasal polyposis was based on the CT scan findings. The condition was classified into three grades, i.e., grade I, II and III. The data was statistically analysed on SPSS version 20.0.</p> <p><strong>Results:</strong> Out of 180 patients diagnosed clinically for nasal polyposis, 97(53.88%) were males. The fungal infestation was positive in 73(41%) specimens. Around 52.72% of patients were found with bilateral involvement. Of which, 31.57% were diagnosed with an allergic event. The fungus <em>Aspergillus</em> was observed in 40.55% of specimens. The one-year follow-up displayed an overall recurrence rate of 40%, i.e., 38(52.05%) patients from the fungal group and 34(47.22%) from the non-fungal group.</p> <p><strong>Conclusion:</strong> It is concluded from the study outcomes that the incidence of nasal polyposis with or without fungal infection is associated with the environment and the discrete host response. Moreover, individuals living with lower socioeconomic conditions in crowded and humid areas tend to suffer more.</p> Mohammad Iqbal, Sahibzada Fawad Khan, Sohail Khan, Wasim Ahmad ##submission.copyrightStatement## Sat, 24 Apr 2021 00:00:00 +0000 Functional recovery of Neurological Deficit among Patients with Orbital Fracture due to Craniofacial Traumas. <p><strong>Background:</strong> With recent development in imaging and bone fixation technology, the orbital fracture is now considered an emerging reconstruction technique for the fixation of even the most severe injuries. This study aims to explore the success rate of functional recovery through different orbital approaches in orbital fractures and reconstruction in patients having craniofacial injuries with clinical and neurological deficits.</p> <p><strong>Methodology:</strong> This observational study was conducted from 2016 to 2018, at the Department of Neurosurgery, Liaquat University of Medical &amp; Health Sciences (LUMHS). A total number of 30 patients were enrolled through consecutive sampling. The patients with any clinical and neurological deficit were included in this study, while the patients with craniofacial fractures with comorbidities were excluded. The evaluation of clinical and neurological deficits in the admitted patients was noted pre-operatively and re-assessment of the same deficit postoperatively was done during the tenure of 1-6 months. All orbital floor fractures were approached via a sub-ciliary or transconjunctival incision with a supraciliary approach used for all supra orbital injuries. The reconstruction of the fracture was done within 5 to 10 days of admission.</p> <p><strong>Results:</strong> A total of 30 patients underwent surgery for the repair of an orbital floor fracture. The mean age observed was 32.09±10.36 years. The patient age range was 20-50 years. Among the patients 22 (73.3.0%) were male and 8(26.7%) patients were female. The improvement of symptoms observed after surgical intervention includes sub-ciliary approach that resides the recovery of diplopia (95%), paraesthesia (75%), enophthalmos (100%) and vertical Dystopia (100%). On the other hand, trans-conjuctival approach resides in the recovery of diplopia (95%), paraesthesia (100%), enophthalmos (100%) and vertical Dystopia (100%).</p> <p><strong>Conclusion:</strong> It is concluded that the surgical treatment of orbital fracture shows promising results in the recovery of neurological deficit among patients with crani-o-facial injuries. Moreover, the trans-conjuctival approach holds much promising results in the improvement of infra-orbital para-aesthesia (100%) as compared to the sub-ciliary approach (75%).</p> Vashdev Khimani, Riaz Ahmed Raja, Ghazala Shahzad, Peer Asad Aziz, Pir Abdul Ahad Aziz Qureshi, Zeeshan Nasir ##submission.copyrightStatement## Wed, 31 Mar 2021 10:09:59 +0000