International Journal of Endorsing Health Science Research (IJEHSR) <p><strong>International journal of endorsing health science research (IJEHSR)</strong>&nbsp;aims to&nbsp;improve health care by providing the most recent research results&nbsp;leading to advancement in health science.</p> <p><strong>IJEHSR</strong>&nbsp;is quarterly, open access, peer-reviewed&nbsp;journal keen to endorse the need for finest healthcare globally, it was founded in 2013 as a biannual journal, and converted into quarterly in 2015. &nbsp;<strong>IJEHSR</strong>&nbsp;is included in the journal list of International Committee of Medical Journal Editors (ICMJE) since 2014. This Journal is indexed by WHO Index Medicus (IMEMR) for the Eastern Mediterranean Region.&nbsp;<strong>IJEHSR</strong>&nbsp;editorial board members are working in diverse fields of health care in countries like&nbsp;<strong>Pakistan, Canada, Australia, United States of America, China, India, Oman,&nbsp;Saudi&nbsp;Arabia,&nbsp;</strong>and<strong>&nbsp;Ethiopia.</strong></p> <p>We would like to encourage you to submit your intellectual effort in&nbsp;IJEHSR, we consider manuscripts from all fields of health sciences.&nbsp;The journal publishes original research, reviews, clinical reports, case studies, legal and policy perspectives.</p> Advance Educational Institute and Research Center en-US International Journal of Endorsing Health Science Research (IJEHSR) 2307-3748 <p>By submitting a manuscript in&nbsp;<strong>IJEHSR</strong>, the Author (Authors if a multi-authored paper) confirms all the clause of the <a href="">Copyright Notice</a></p> Antibiotic susceptibility and antibacterial activity of neutralized cell-free supernatant of Lactobacillus rhamnosus MT539286 against Foodborne and Clinical pathogens. <p><strong>Background: </strong>The emergence of horizontally transferable antibiotic resistance (AR) in probiotic strains of lactic acid bacteria (LAB) has incited a need to establish stringent safety measures for the assessment of commercial probiotics. <em>Lactobacillus rhamnosus</em> is not only a prevalent constituent of commercially available probiotics but also has an abundant presence in fermented foods.</p> <p><strong>Methodology:</strong>&nbsp; The present study tested antimicrobial activity and antibiotic susceptibility of <em>L. rhamnosus</em> isolated from fermented cabbage. Agar well diffusion assay was used to determine the antibacterial activity against a number of Gram-positive and Gram-negative bacteria, and antibiotic susceptibility testing was used to determine the antibiogram of several antibiotics. For this, the neutralized Cell-Free Supernatant (nCFS) of <em>L. rhamnosus</em> was used, which exhibited antibacterial activity against several bacteria.&nbsp;</p> <p><strong>Results: </strong>Antagonistic activity of nCFS of <em>L. rhamnosus</em> was found against <em>Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Salmonella typhimurium, Klebsiella pneumoniae, Salmonella enterica</em>. Maximum inhibitory activity was observed against <em>Listeria monocytogenes</em>. Whereas, no activity was found against <em>Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtilis, Vibrio alginolyticus, Proteus mirabilis</em> and <em>Proteus vulgaris</em>. The intensity of inhibition among the isolates was in the sequence of <em>Listeria monocytogenes</em> &gt; <em>Escherichia coli</em> &gt; <em>Bacillus cereus</em> &gt; <em>Vibrio parahaemolyticus</em> &gt; <em>Salmonella typhimurium</em>. <em>L. rhamnosus</em> was found susceptible against Chloramphenicol and Novobiocin. Moreover, it exhibited resistance to Amoxicillin, Levofloxacin, Oxacillin, Streptomycin and Tobramycin.</p> <p><strong>Conclusion:</strong> &nbsp;This study suggests that the use of <em>L. rhamnosus</em> as a probiotic requires extensive examination of AR genes present in this strain.</p> Muffrah Hasan Afsheen Arif Abid Hasnain Tanveer Abbas ##submission.copyrightStatement## 2020-12-21 2020-12-21 Assessing the impact of Glutathione on maternal and fetal outcome in pregnancy-induced hypertensive disorders: A case-control study <p><strong>Background: </strong>Disorders related to hypertension in pregnancy, mainly Pre-eclampsia (PE), and Eclampsia, are the major causes of fetal and maternal morbidity and mortality. The placenta's ischemic blood supply leads to its endothelial dysfunction and reduced glutathione bioavailability involved in its pathogenesis. This study explored maternal serum Glutathione's level and changes and found out its association with fetal growth in pregnancy-induced hypertensive disorders.</p> <p><strong>Methodology:</strong> A Multicenter, case-control study was conducted on 240 pregnant females. The investigational group included 180 pregnant females having blood pressure above 140/90 mmHg. The Investigational group was divided into different groups, i.e. Pregnancy-induced hypertension (PIH) group, PE group, and Eclamptic group.&nbsp; 60 normotensive pregnant females were kept in control. The blood samples were obtained to analyze serum Glutathione (GSH) through ELIZA (enzyme-linked immunosorbent assay), while urine samples were obtained for confirmation of the PE status. Fetal well-being and signs of growth restriction were observed using ultrasound.</p> <p><strong>Results: </strong>A significant reduction was elucidated in serum glutathione, biparietal diameter, and femur length among all experimental groups p &lt; 0.001. However, no significant difference was observed in the abdominal circumference p=0.122 and Fetal weight p=0.51. A significantly inversely proportional relation was found between serum glutathione and gestational age, fetal weight as well as head circumference in all four groups control (r =-.305 p=0.018) PIH (r =-.618 p =0.000), PE (r=-.707 p=0.000) and Eclampsia (r=-.661 p=0.000).</p> <p><strong>Conclusion:</strong> Study revealed that the fetus's growth was markedly affected by reduced Glutathione in hypertensive disorders of pregnancy with the progression of the disease.</p> Sonya Arshad Sadaf Ahmed Shershah Syed ##submission.copyrightStatement## 2020-12-24 2020-12-24 Assessment of the association of ABO blood group in dengue fever diagnosed patient in tertiary care hospital <p><strong>Background:&nbsp;</strong>A serious worldwide medical and public health concern occurs with the dengue virus, which is also an emerging health threat. A victim can be vulnerable and acquire dengue because of his age, genetics, nutritional status, viral strain and secondary infections. Our objective was to identify the ABO blood group system's distribution and relationship in dengue fever (DF) patients.</p> <p><strong>Methodology: </strong>The current study has conducted between September 2019 and September 2020 in Dr. Ishrat ul Ebad Institute of Blood Diseases Department. The research work was viewed and accepted by the ethics and research committees. Case records of patients with blood systems and their relation among dengue nonstructural protein 1 (NS1) antigen /dengue immunoglobulin M (IgM) and immunoglobulin G (lgG) were included in this study.</p> <p><strong>Results:</strong> Among 204 cases, 82 were blood group O positive, 59 were B positive, 38 were A positive, 16 were AB positive, 4 were O negative, and 2 were blood group A negative. The association of the ABO blood group with dengue was statistically significant (p=0.046). Blood group O+ has the highest number of cases, 66 (42.9 %), than controls, 16 (32 %).</p> <p><strong>Conclusion:</strong> This study overall consummates that blood group O is linked with a greater possibility of acquiring DF in comparison with different other blood group types. There is a significant relationship between blood group and DF observed.</p> Shaheen Kouser Kouser Farah Fatima Abbas Fouzia Naeem Effendi Nasrul Huda Munazza Suharwardy Obaid Humaira Aman Ali Muhammad Imran Aabiya Abdus Salam Alifya Murtaza Ali Mahrukh Nazneen Suri Syeda Fatima Munir ##submission.copyrightStatement## 2020-12-30 2020-12-30 Toxicity evaluations of Papaya seed extract administration in Albino Wistar rats. <p><strong>Background:</strong> Carica papaya seeds are regarded as waste or by-product of the fruit since it is the inedible part of the plant. However, it holds a diversified range of phytochemicals that anticipate their therapeutic role in different types of ailments. The seed extract has been proven for the presence of multiple health benefiting contents and some toxic agents. Studies evaluating the toxicity of the Papaya seed extract are still scarce to support its safe use as a therapeutic agent. Therefore, the undertaken study is to explore the safe dose of the aqueous extract of Papaya seeds.</p> <p><strong>Methodology:</strong> This study is designed to examine the toxic potential of Papaya seed aqueous extract on Wistar rats for 42 days by oral administration at dose regimens of 100, 200 and 400 mg/kg body weight in the liver. The groups (n=6) allotted include control untreated and group I (100 mg/kg), group II (200 mg/kg) and group III (400 mg/kg). The liver toxicity was assessed using liver enzymes as toxicity markers. The morphology of liver cells was observed by histology.</p> <p><strong>Results:</strong> Our results showed no mortality or signs of toxicity. The body weights were observed to be declining with increasing doses. However, other biochemical parameters displayed no significant alterations in treated groups as compared to the controls.</p> <p><strong>Conclusion:</strong> This study supports the evidence that chosen doses of aqueous extract of papaya seeds are safe for normal liver functioning. This evidence endorses the use of the extracts of the seeds as a therapeutic intervention.</p> Nazneen Zehra Lubna Naz ##submission.copyrightStatement## 2021-01-04 2021-01-04